Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CYP3A4 Antibody in NCI-H460 cell line lysates (35ug/lane)Application
CYP3A4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysates from HepG2, A549, HT-29 cell line, human kidney and liver tissue lysate (from left to right), using CYP3A4 Antibody at 1:1000 at each lane.Application
Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody at 1:1000.| Product Name | CYP3A4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.1) Sandanaraj,E., Clin. Cancer Res. 14 (21), 7116-7126 (2008) |
| Immunogen | This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CYP3A4 |
|---|---|
| Alternative Names | Cytochrome P450 3A4 11413- 8-cineole 2-exo-monooxygenase Albendazole monooxygenase Albendazole sulfoxidase CYPIIIA3 CYPIIIA4 Cytochrome P450 3A3 Cytochrome P450 HLp Cytochrome P450 NF-25 Cytochrome P450-PCN1 Nifedipine oxidase Quinine 3-monooxyg |
| Molecular Weight(MW) | 57 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CYP3A4 Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
CYP3A4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysates from HepG2, A549, HT-29 cell line, human kidney and liver tissue lysate (from left to right), using CYP3A4 Antibody at 1:1000 at each lane.
Application
Western blot analysis of lysate from human ovary tissue, using CYP3A4 Antibody at 1:1000.| Application Notes | For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
