Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP3A5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CYP3A5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.1) Zencir,S., Z. Naturforsch., C, J. Biosci. 63 (9-10), 780-784 (2008) |
| Immunogen | This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-218 amino acids from the Central region of human CYP3A5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CYP3A5 |
|---|---|
| Alternative Names | Cytochrome P450 3A5 CYPIIIA5 Cytochrome P450 HLp2 Cytochrome P450-PCN3 CYP3A5 |
| Molecular Weight(MW) | 57 kDa |
Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP3A5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
