Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CYP4F3 Antibody in K562 cell line lysates (35ug/lane)| Product Name | CYP4F3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Docosahexaenoic acid omega-hydroxylase CYP4F3, 20-hydroxyeicosatetraenoic acid synthase, 20-HETE synthase, 11413-, CYPIVF3, Cytochrome P450 4F3, Cytochrome P450-LTB-omega, Leukotriene-B(4) 20-monooxygenase 2, Leukotriene-B(4) omega-hydroxylase 2, CYP4F3, L |
| Product description | CYP4F3 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation.1) Kikuta,Y., et.al., FEBS Lett. 348 (1), 70-74 (1994) |
| Immunogen | This CYP4F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CYP4F3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CYP4F3 |
|---|---|
| Alternative Names | Docosahexaenoic acid omega-hydroxylase CYP4F3 20-hydroxyeicosatetraenoic acid synthase 20-HETE synthase 11413- CYPIVF3 Cytochrome P450 4F3 Cytochrome P450-LTB-omega Leukotriene-B(4) 20-monooxygenase 2 Leukotriene-B(4) omega-hydroxylase 2 CYP4F3 L |
| Molecular Weight(MW) | 60 kDa |
Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CYP4F3 Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
