Application
Western blot analysis of CYP51A1 Antibody in HL-60 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CYP51A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.1) Wang,Y., et.al., J. Biol. Chem. 283 (39), 26332-26339 (2008) |
| Immunogen | This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human CYP51A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CYP51A1 |
|---|---|
| Alternative Names | Lanosterol 14-alpha demethylase LDM CYPLI Cytochrome P450 51A1 Cytochrome P450-14DM Cytochrome P45014DM Cytochrome P450LI Sterol 14-alpha demethylase CYP51A1 CYP51 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of CYP51A1 Antibody in HL-60 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
