Application
The immunogen is horse cytochrome c. Schematic structure of horse cytochrome c (aa. 1-104) as seen in this image.| Product Name | Cytochrome c Antibody [SJL2-4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 1) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997). |
| Immunogen | Cytochrome C (monoclonal SJL2-4) antibody was raised against Horse cytochrome c. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB |
| Cytochrome C antibody can be used for detection of horse cytochrome c by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apocytochrome c. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system. | |
| Species Reactivity | GoatHumanMonkeyMouseRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYCS |
|---|---|
| Alternative Names | CYC Cytochrome c |
Application
The immunogen is horse cytochrome c. Schematic structure of horse cytochrome c (aa. 1-104) as seen in this image.| Application Notes | Cytochrome C antibody can be used for detection of horse cytochrome c by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apocytochrome c. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Cytochrome c antibody can be stored at 4˚C, stable for one year. |
| Storage Buffer | PBS containing 0.02% sodium azide |
