Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. In the development of Drosophila, SUMO plays a maternal role in anterior-posterior (A/P) polarity and patterning.1) Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review.
Immunogen
This Drosophila SUMO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the C-terminal region of human Drosophila SUMO.
Antibody is used in Western blot to detect SUMO1 in HL-60 cell lysate.
Application
Western blot analysis of lysate from Drosophila tissue lysate, using SUMO Antibody (V52)(Drsophila) at 1:1000.
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.