Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).| Product Name | DAAM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.1) Liu,W., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 210-215 (2008) |
| Immunogen | This DAAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1034-1063 amino acids from the C-terminal region of human DAAM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAAM1 |
|---|---|
| Alternative Names | Disheveled-associated activator of morphogenesis 1 DAAM1 KIAA0666 |
| Molecular Weight(MW) | 123 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of DAAM1 using rabbit polyclonal DAAM1 Antibody(Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
