Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).Application
DAAM1-T361 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).| Product Name | DAAM1-T361 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.1) Higashi, T., et al. J. Biol. Chem. 283(13):8746-8755(2008) |
| Immunogen | This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the N-terminal region of human DAAM1-T361. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAAM1 |
|---|---|
| Alternative Names | Disheveled-associated activator of morphogenesis 1 DAAM1 KIAA0666 |
| Molecular Weight(MW) | 123 kDa |
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).
Application
DAAM1-T361 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
