Application
Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 μg/mL.Application
Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 μg/mL.Application
Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 μg/mL.| Product Name | Dact2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Dact2 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.1) Shimigori T, VanSant J, Paik E, et al. Members of the Wnt, Fz, and Frp gene families expressed in postnatal mouse cerebral cortex. J. Comp. Neurol.2004; 473:496-510. |
| Immunogen | Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2. The immunogen is located within amino acids 150 - 200 of Dact2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DACT2 |
|---|---|
| Alternative Names | Dact2 Antibody: DPR2 DAPPER2 C6orf116 bA503C24.7 PP13671 Dapper homolog 2 Dapper antagonist of catenin 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 μg/mL.
Application
Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 μg/mL.
Application
Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 μg/mL.| Application Notes | Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Dact2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Dact2 Antibody is supplied in PBS containing 0.02% sodium azide. |
