Application
Western blot analysis of Dact3 in rat brain tissue lysate with Dact3 antibody at 1 μg/mL.| Product Name | Dact3 Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Dact3 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact3, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact3 is expressed in the ventral region of maturing somites, limb bud and branchial arch mesenchyme, embryonic CNS, and the adult brain. Recent evidence shows that Dact3 acts as a negative regulator Wnt/beta-catenin signaling that is repressed at the transcriptional level in colorectal cancer and this repression is associated with bivalent histone modifications. This repression can be reversed by pharmacological agents that targets both histone methylation and deacetylation, suggesting that Dact3 may be a potential target for therapeutic treatment of this cancer. At least three isoforms of Dact3 are known to exist.1) Shimigori T, VanSant J, Paik E, et al. Members of the Wnt, Fz, and Frp gene families expressed in postnatal mouse cerebral cortex. J. Comp. Neurol.2004; 473:496-510. |
| Immunogen | Dact3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Dact3. The immunogen is located within amino acids 370 - 420 of Dact3. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Dact3 antibody can be used for detection of Dact3 by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DACT3 |
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| Alternative Names | Dact3 Antibody: RRR1 DAPPER3 RRR1 Dapper homolog 3 Antagonist of beta-catenin Dapper homolog 3 |
Application
Western blot analysis of Dact3 in rat brain tissue lysate with Dact3 antibody at 1 μg/mL.| Application Notes | Dact3 antibody can be used for detection of Dact3 by Western blot at 1 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | Dact3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Dact3 Antibody is supplied in PBS containing 0.02% sodium azide. |
