Application
Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.Application
Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.Application
Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.| Product Name | DAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).1) Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44. |
| Immunogen | DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD55 |
|---|---|
| Alternative Names | Decay-accelerating factor CD55 molecule decay accelerating factor for complement (Cromer blood group) CD55 CR TC CROM |
| Molecular Weight(MW) | Predicted: 48 kDa Observed: 49 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform. |
| Entrez Gene | 1604 |
|---|---|
| Protein Accession | NP_001108224 |
Application
Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
Application
Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
Application
Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.| Application Notes | DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DAF antibody is supplied in PBS containing 0.02% sodium azide. |
