Application
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
DAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | DAG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000-1:2000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAG1 |
|---|---|
| Alternative Names | Dystroglycan Dystrophin-associated glycoprotein 1 Alpha-dystroglycan Alpha-DG Beta-dystroglycan Beta-DG DAG1 |
| Molecular Weight(MW) | 97 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit |
Application
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
DAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000-1:2000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
