Application
Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.Application
Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.Application
Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.| Product Name | DAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).1) Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30. |
| Immunogen | DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAP |
|---|---|
| Alternative Names | DAP Antibody: DAP1 Death-associated protein 1 DAP-1 |
| Molecular Weight(MW) | Predicted: 11 kDa Observed: 11 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | DAP antibody is human, mouse and rat reactive. |
Application
Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
Application
Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.
Application
Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.| Application Notes | DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | DAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DAP Antibody is supplied in PBS containing 0.02% sodium azide. |
