WB
Western blot analysis of DAP using anti-DAP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: U-937 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: PC-3 whole cell lysates, Lane 4: Hela whole cell lysates, Lane 5: HepG2 whole cell lysates, Lane 6: U2OS whole cell lysates, Lane 7: HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DAP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of DAP using anti-DAP antibody. DAP was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DAP Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of DAP using anti-DAP antibody. DAP was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-DAP Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HL-60 cells using anti-DAP antibody. Overlay histogram showing HL-60 cells stained with anti-DAP antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-DAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DAP1/DAP, which shares 92.9% amino acid (aa) sequence identity with both mouse and rat DAP1/DAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAP |
|---|---|
| Gene Synonyms | DAP1 |
| Gene Full Name | death associated protein |
| Gene Summary | This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Alternative Names | DAP DAP1 Death-associated protein 1 DAP-1 |
| Molecular Weight(MW) | 11kDa(Observed MW 15kDa) |
WB
Western blot analysis of DAP using anti-DAP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: U-937 whole cell lysates, Lane 2: RT4 whole cell lysates, Lane 3: PC-3 whole cell lysates, Lane 4: Hela whole cell lysates, Lane 5: HepG2 whole cell lysates, Lane 6: U2OS whole cell lysates, Lane 7: HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DAP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of DAP using anti-DAP antibody. DAP was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DAP Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of DAP using anti-DAP antibody. DAP was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-DAP Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HL-60 cells using anti-DAP antibody. Overlay histogram showing HL-60 cells stained with anti-DAP antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
