Application
Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.Application
Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.| Product Name | DARC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DARC Antibody: DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.1) Chaudhuri A, Polyakova J, Zbrzezna V, et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA1993; 90:10793-7. |
| Immunogen | DARC antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC. The immunogen is located within the last 50 amino acids of DARC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DARC antibody can be used for detection of DARC by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DARC |
|---|---|
| Alternative Names | DARC Antibody: FY Dfy GPD GpFy ACKR1 CCBP1 CD234 WBCQ1 FY Atypical chemokine receptor 1 |
Application
Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.
Application
Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.| Application Notes | DARC antibody can be used for detection of DARC by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | DARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DARC Antibody is supplied in PBS containing 0.02% sodium azide. |
