Application
Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Skin at 4.0-8.0 ug/ml.| Product Name | DAZ2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DAZ2 is a member of the DAZ family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). This RNA-binding protein is important for spermatogenesis.This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Kuo, P.L., (2004) Fertil. Steril. 81 (4), 1034-1040. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DAZ2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| DAZ2 antibody can be used for detection of DAZ2 by ELISA at 1:62500. DAZ2 antibody can be used for detection of DAZ2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAZ2 |
|---|---|
| Alternative Names | DAZ2 pDP1678 |
| Molecular Weight(MW) | 59 kDa, 65 kDa, 65 kDa, 41 kDa, 65 kDa, 65 kDa, 63 kDa, 50 kDa, 44 kDa |
| Entrez Gene | 57055 |
|---|---|
| Protein Accession | NP_001005785 |
Application
Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Skin at 4.0-8.0 ug/ml.| Application Notes | DAZ2 antibody can be used for detection of DAZ2 by ELISA at 1:62500. DAZ2 antibody can be used for detection of DAZ2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DAZ2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
