Application
Immunofluorescence of DBX1 in human kidney tissue with DBX1 antibody at 20 μg/mL.Application
Immunohistochemistry of DBX1 in human kidney tissue with DBX1 antibody at 2.5 μg/mL.Application
Western blot analysis of DBX1 in mouse kidney tissue lysate with DBX1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DBX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DBX1 Antibody: DBX1 is implicated in patterning the central nervous system during embryogenesis. DBX1 homeodomain transcription factor is expressed in progenitors which are critical in establishing cell fate allocation and cell diversity. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. DBX1-derived neurons are essential for the transgenic expression and generation of respiratory behavior both in vitro and in vivo.1) Griveau A, Borello U, Causeret F, et al. A novel role for Dbx1-derived Cajal-Retzius cells in early regionalization of the cerebral cortical neuroepithelium. PLoS Biol. 2010; 8(7):e1000440 |
| Immunogen | DBX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human DBX1. The immunogen is located within amino acids 100 - 150 of DBX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DBX1 |
|---|---|
| Alternative Names | DBX1 Antibody: Homeobox protein DBX1 Developing brain homeobox protein 1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | BX1 antibody is predicted to not cross-react with other DBX protein family members |
| Entrez Gene | 120237 |
|---|---|
| Protein Accession | NP_001025036 |
Application
Immunofluorescence of DBX1 in human kidney tissue with DBX1 antibody at 20 μg/mL.
Application
Immunohistochemistry of DBX1 in human kidney tissue with DBX1 antibody at 2.5 μg/mL.
Application
Western blot analysis of DBX1 in mouse kidney tissue lysate with DBX1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | DBX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DBX1 Antibody is supplied in PBS containing 0.02% sodium azide. |
