Application
Immunohistochemistry of DC-SIGN in human small intestine tissue with DC-SIGN antibody at 10 μg/mL.Application
Western blot analysis of DC-SIGN expression in human placenta tissue lysate in the absence (lane 1) and presence (lane 2) of blocking peptide with DC-SIGN antibody at 2 μg /ml.| Product Name | DC-SIGN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DC-SIGN Antibody: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, CD209 antigen, C-type lectin domain family 4 member L |
| Product description | DC-SIGN Antibody: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.1) Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000;100:587-97. |
| Immunogen | DC-SIGN antibody was raised against a synthetic peptide corresponding to 17 amino acids near the center of human DC-SIGN. The immunogen is located within amino acids 250 - 300 of DC-SIGN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DC-SIGN antibody can be used for detection of DC-SIGN by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD209 |
|---|---|
| Alternative Names | DC-SIGN Antibody: CDSIGN CLEC4L DC-SIGN DC-SIGN1 CD209 antigen C-type lectin domain family 4 member L |
| Molecular Weight(MW) | Predicted: 44 kDa Observed: 47 kDa |
| Sequence Similarities | 3) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
Application
Immunohistochemistry of DC-SIGN in human small intestine tissue with DC-SIGN antibody at 10 μg/mL.
Application
Western blot analysis of DC-SIGN expression in human placenta tissue lysate in the absence (lane 1) and presence (lane 2) of blocking peptide with DC-SIGN antibody at 2 μg /ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Tissue Lysate |
|---|---|
| Application Notes | DC-SIGN antibody can be used for detection of DC-SIGN by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DC-SIGN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DC-SIGN Antibody is supplied in PBS containing 0.02% sodium azide. |
