Application
DCC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Product Name | DCC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This DCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-670 amino acids from the Central region of human DCC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCC |
|---|---|
| Alternative Names | Netrin receptor DCC Colorectal cancer suppressor Immunoglobulin superfamily DCC subclass member 1 Tumor suppressor protein DCC DCC IGDCC1 |
| Molecular Weight(MW) | 158 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
DCC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
