Application
Western Blot at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of DCI Antibody in MCF-7, 293 cell line lysates (35ug/lane)| Product Name | DCI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Enoyl-CoA delta isomerase 1, mitochondrial,2-trans-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI |
| Product description | DCI encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.1) Partanen,S.T., et.al., J. Mol. Biol. 342 (4), 1197-1208 (2004) |
| Immunogen | This DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECI1 |
|---|---|
| Alternative Names | Enoyl-CoA delta isomerase 1 mitochondrial 2-trans-enoyl-CoA isomerase Delta(3) Delta(2)-enoyl-CoA isomerase D3 D2-enoyl-CoA isomerase Dodecenoyl-CoA isomerase ECI1 DCI |
| Molecular Weight(MW) | 33 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of DCI Antibody in MCF-7, 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
