Application
Immunohistochemistry of DLCK2 in rat brain tissue with DLCK2 antibody at 5 μg/mL.Application
Western blot analysis of DCLK2 in rat brain tissue lysate with DCLK2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DCLK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCLK2 Antibody: CL2, DCK2, CLIK2, DCDC3, CLICK2, DCDC3B, DCAMKL2, CLICK-II, CaMK-like CREB regulatory kinase 2, CL2 |
| Product description | DCLK2 Antibody: DCLK2 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK2 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.1) Sossey-Alaoui K and Srivastava AK. DCAMKL1, a brain specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX), Genomics1999; 56:121-6. |
| Immunogen | DCLK2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK2. The immunogen is located within the last 50 amino acids of DCLK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DCLK2 antibody can be used for detection of DCLK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCLK2 |
|---|---|
| Alternative Names | DCLK2 Antibody: CL2 DCK2 CLIK2 DCDC3 CLICK2 DCDC3B DCAMKL2 CLICK-II CaMK-like CREB regulatory kinase 2 CL2 |
| Molecular Weight(MW) | Predicted: 85 kDa Observed: 85 kDa |
| Entrez Gene | 166614 |
|---|---|
| Protein Accession | NP_001035350 |
Application
Immunohistochemistry of DLCK2 in rat brain tissue with DLCK2 antibody at 5 μg/mL.
Application
Western blot analysis of DCLK2 in rat brain tissue lysate with DCLK2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1413 – Mouse Cerebrum Tissue Lysate |
|---|---|
| Application Notes | DCLK2 antibody can be used for detection of DCLK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DCLK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DCLK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
