Application
Immunohistochemistry of DCNP1 in mouse skeletal muscle tissue with DCNP1 antibody at 5 μg/ml.Application
Immunofluorescence of DCNP1 in mouse skeletal muscle tissue with DCNP1 antibody at 20 μg/ml.Application
Western blot analysis of DCNP1 in mouse skeletal muscle tissue lysate with DCNP1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | DCNP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCNP1 Antibody: DCNP1, C5orf20, Dendritic cell nuclear protein 1 |
| Product description | The DCNP1 (dendritic cell nuclear protein 1) gene is specifically expressed in dendritic cells, potent antigen-presenting cells involved in activating naive T cells to initiate antigen-specific immune response (1). It is localized mainly in the perinucleus. One of the alleles (A/T) of this gene causes premature translation termination at aa 117 and has been associated with an increased prevalence of major depression in humans (2,3). DCNP1 may play a role in the pathogenesis of depressive disorders by enhancing corticotropin-releasing hormone expression in the hypothalamic paraventricular nucleus (4).1) Masuda M, Senju S, Fujii Si S, et al. Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein. Biochem. Biophys. Res. Commun. 2002; 290:1022-9. |
| Immunogen | DCNP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human DCNP1. The immunogen is located within the first 50 amino acids of DCNP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DCNP1 antibody can be used for detection of DCNP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C5orf20 |
|---|---|
| Alternative Names | DCNP1 Antibody: DCNP1 C5orf20 Dendritic cell nuclear protein 1 |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 24 kDa |
| Tissue Specificity | DCNP1 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of DCNP1 in mouse skeletal muscle tissue with DCNP1 antibody at 5 μg/ml.
Application
Immunofluorescence of DCNP1 in mouse skeletal muscle tissue with DCNP1 antibody at 20 μg/ml.
Application
Western blot analysis of DCNP1 in mouse skeletal muscle tissue lysate with DCNP1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | DCNP1 antibody can be used for detection of DCNP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DCNP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DCNP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
