Application
Immunofluorescence of DCP2 in human testis tissue with DCP2 antibody at 20 μg/mL.Application
Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | DCP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCP2 Antibody: NUDT20, NUDT20, m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20 |
| Product description | DCP2 Antibody: The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.1) Song MG, Li Y, and Kiledjian M. Multiple mRNA decapping enzymes in mammalian cells. Mol. Cell 2010; 40:423-32. |
| Immunogen | DCP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DCP2. The immunogen is located within amino acids 340 - 390 of DCP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| DCP2 antibody can be used for detection of DCP2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCP2 |
|---|---|
| Alternative Names | DCP2 Antibody: NUDT20 NUDT20 m7GpppN-mRNA hydrolase Nucleoside diphosphate-linked moiety X motif 20 Nudix motif 20 |
| Molecular Weight(MW) | 46 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | Multiple isoforms of DCP2 are known to exist. |
Application
Immunofluorescence of DCP2 in human testis tissue with DCP2 antibody at 20 μg/mL.
Application
Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | DCP2 antibody can be used for detection of DCP2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DCP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DCP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
