Application
Immunohistochemistry of DcR3 in human heart tissue with DcR3 antibody at 1 μg/mL.Application
Western blot analysis of DcR3 in human heart (H), brain (B), and kidney (K) tissue lysates with DcR3 antibody at 1:500 dilution.| Product Name | DcR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DcR3 Antibody: M68, TR6, DCR3, M68E, DJ583P15.1.1, UNQ186/PRO212, Tumor necrosis factor receptor superfamily member 6B, Decoy receptor 3, DcR3 |
| Product description | DcR3 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. Several novel members in the TNFR family including DR3, DR4, DR5, and DR6 were recently discovered and function as cell death receptors. Two decoy receptors, DcR1 and DcR2, were recently identified to compete with DR4 and DR5 for their ligand TRAIL binding. A novel decoy receptor was more recently discovered and designated DcR3 and TR6, respectively,. Unlike DcR1 and DcR2, DcR3 is a soluble rather than a membrane associated molecule. DcR3 binds to FasL and LIGHT and inhibits FasL and LIGHT induced apoptosis. DcR3 transcript is expressed in a number of lung and colon carcinomas and in some normal tissues.1) Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, Huang A, Donahue CJ, Sherwood SW, Baldwin DT, Godowski PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD, Botstein D, Ashkenazi A. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699-703 |
| Immunogen | DcR3 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DcR3 precursor. The immunogen is located within the first 50 amino acids of DcR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DcR3 antibody can be used for detection of DcR3 expression by Western blot 0.5 μg/mL. An approximately 33 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF6B |
|---|---|
| Alternative Names | DcR3 Antibody: M68 TR6 DCR3 M68E DJ583P15.1.1 UNQ186/PRO212 Tumor necrosis factor receptor superfamily member 6B Decoy receptor 3 DcR3 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunohistochemistry of DcR3 in human heart tissue with DcR3 antibody at 1 μg/mL.
Application
Western blot analysis of DcR3 in human heart (H), brain (B), and kidney (K) tissue lysates with DcR3 antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | DcR3 antibody can be used for detection of DcR3 expression by Western blot 0.5 μg/mL. An approximately 33 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DcR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DcR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
