Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of DCT Antibody in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane)| Product Name | DCT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | L-dopachrome tautomerase, DCT, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2, DCT, TYRP2 |
| Product description | DCT is involved in regulating eumelanin and phaeomelanin levels.1) Nishihira,J., et.al., Biochem. Biophys. Res. Commun. 243 (2), 538-544 (1998) |
| Immunogen | This DCT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the N-terminal region of human DCT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCT |
|---|---|
| Alternative Names | L-dopachrome tautomerase DCT DT L-dopachrome Delta-isomerase Tyrosinase-related protein 2 TRP-2 TRP2 DCT TYRP2 |
| Molecular Weight(MW) | 59 kDa |
Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of DCT Antibody in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM276813