Application
Immunohistochemistry of DDA1 in mouse heart tissue with DDA1 antibody at 5 μg/mL.Application
Immunofluorescence of DDA1 in mouse heart tissue with DDA1 antibody at 20 μg/mL.Application
Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | DDA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDA Antibody: PCIA1, C19orf58, PCIA1, DET1- and DDB1-associated protein 1, Placenta cross-immune reaction antigen 1, PCIA-1 |
| Product description | DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.1) Pick E, Lau O, Tsuge T, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol. Cell. Biol. 2007; 27:4708-19. |
| Immunogen | DDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DDA1. The immunogen is located within the last 50 amino acids of DDA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DDA1 antibody can be used for detection of DDA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDA1 |
|---|---|
| Alternative Names | DDA Antibody: PCIA1 C19orf58 PCIA1 DET1- and DDB1-associated protein 1 Placenta cross-immune reaction antigen 1 PCIA-1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Immunohistochemistry of DDA1 in mouse heart tissue with DDA1 antibody at 5 μg/mL.
Application
Immunofluorescence of DDA1 in mouse heart tissue with DDA1 antibody at 20 μg/mL.
Application
Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | DDA1 antibody can be used for detection of DDA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DDA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DDA Antibody is supplied in PBS containing 0.02% sodium azide. |
