Application
Confocal immunofluorescent analysis of DDAH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of DDAH1 Antibody in mouse kidney tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | DDAH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, DDAHI, Dimethylargininase-1, DDAH1, DDAH |
| Product description | DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.1) Ellger,B., et.al., Endocrinology 149 (6), 3148-3157 (2008) |
| Immunogen | This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DDAH1 |
|---|---|
| Alternative Names | N(G) N(G)-dimethylarginine dimethylaminohydrolase 1 DDAH-1 Dimethylarginine dimethylaminohydrolase 1 DDAHI Dimethylargininase-1 DDAH1 DDAH |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Confocal immunofluorescent analysis of DDAH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of DDAH1 Antibody in mouse kidney tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
