Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Product Name | DDOST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDOST, AGE-R1, KIAA0115, MGC2191, OK/SW-cl.45, OST, OST48, WBP1, AGER1, CDG1R, OKSWcl45 |
| Product description | DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.1) Shibatani, T., (2005) Biochemistry 44 (16), 5982-5992. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DDOST |
|---|---|
| Alternative Names | DDOST AGE-R1 KIAA0115 MGC2191 OK/SW-cl.45 OST OST48 WBP1 AGER1 CDG1R OKSWcl45 |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDOST antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
