Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | DDX11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, hCHLR1, DEAD/H box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1, CHLR1, KRG2 |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms.1) Leman, A.R., et al. J. Cell. Sci. 123 (PT 5), 660-670 (2010) : |
| Immunogen | This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-847 amino acids from the C-terminal region of human DDX11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX11 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX11 CHL1-related protein 1 hCHLR1 DEAD/H box protein 11 Keratinocyte growth factor-regulated gene 2 protein KRG-2 DDX11 CHL1 CHLR1 KRG2 |
| Molecular Weight(MW) | 108 kDa |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
