Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Product Name | DDX23 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX23, prp28, PRPF28, U5-100K, SNRNP100, U5-100KD |
| Product description | DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.1) Teigelkamp, S., et al., (1997) RNA 3 (11), 1313-1326. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX23. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDX23 antibody can be used for detection of DDX23 by ELISA at 1:1562500. DDX23 antibody can be used for detection of DDX23 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DDX23 |
|---|---|
| Alternative Names | DDX23 prp28 PRPF28 U5-100K SNRNP100 U5-100KD |
| Molecular Weight(MW) | 90 kDa |
Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DDX23 antibody can be used for detection of DDX23 by ELISA at 1:1562500. DDX23 antibody can be used for detection of DDX23 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX23 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
