Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of DDX23 Antibody in 293 cell line lysates (35ug/lane)Application
DDX23 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | DDX23 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23 |
| Product description | DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.1) Mathew, R., et al. Nat. Struct. Mol. Biol. 15(5):435-443(2008) |
| Immunogen | This DDX23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-277 amino acids from the Central region of human DDX23. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX23 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX23 100 kDa U5 snRNP-specific protein DEAD box protein 23 PRP28 homolog U5-100kD DDX23 |
| Molecular Weight(MW) | 96 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of DDX23 Antibody in 293 cell line lysates (35ug/lane)
Application
DDX23 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
