Application
Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.Application
Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.Application
Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | DDX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX3 Antibody: DBX, DDX3, HLP2, DDX14, DBX, ATP-dependent RNA helicase DDX3X, DEAD box protein 3, X-chromosomal |
| Product description | DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.1) Linder P, Lasko PF, Ashburner M, et al. Birth of the D-E-A-D box. Nature 1989; 337:121-2. |
| Immunogen | DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3. The immunogen is located within amino acids 110 - 160 of DDX3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX3X |
|---|---|
| Alternative Names | DDX3 Antibody: DBX DDX3 HLP2 DDX14 DBX ATP-dependent RNA helicase DDX3X DEAD box protein 3 X-chromosomal |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | DDX3 antibody will detect both DDX3 and DBY. |
Application
Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.
Application
Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.
Application
Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DDX3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DDX3 Antibody is supplied in PBS containing 0.02% sodium azide. |
