Immunohistochemical
Human brain stem cells (NT2) Primary Antibody Dilution : 1:500Secondary Antibody :Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :Red: DDX3X Blue: DAPIGene Name :DDX3XSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Sample Type: 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: THP-1 cell lysate| Product Name | Rabbit anti-DDX3X polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DBX, DDX14, DDX3, HLP2 |
| Immunogen | The immunogen for anti-DDX3X antibody: synthetic peptide directed towards the N terminal of human DDX3X |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX3X |
|---|---|
| Gene Synonyms | DBX DDX14 DDX3 HLP2 |
| Gene Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and m |
| Alternative Names | DBX DDX14 DDX3 HLP2 |
| Molecular Weight(MW) | 73kDa |
| Sequence | 662 amino acids |
Immunohistochemical
Human brain stem cells (NT2) Primary Antibody Dilution : 1:500Secondary Antibody :Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :Red: DDX3X Blue: DAPIGene Name :DDX3XSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Sample Type: 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: THP-1 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
