Application
Western blot analysis in WiDr cell line lysates (35ug/lane).Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).| Product Name | DDX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.1) Tilgner, K., et al. Stem Cells 28(1):84-92(2010) |
| Immunogen | This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human DDX4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX4 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX4 DEAD box protein 4 Vasa homolog DDX4 VASA |
| Molecular Weight(MW) | 79 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).
Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
