Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.| Product Name | DDX42 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX42, FLJ43179, RHELP, RNAHP, SF3b125, SF3B8, DDX42P |
| Product description | DDX42 is a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX42 is a ATP-dependent RNA helicase. DDX42 binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. It also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein.This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX42. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDX42 antibody can be used for detection of DDX42 by ELISA at 1:1562500. DDX42 antibody can be used for detection of DDX42 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX42 |
|---|---|
| Alternative Names | DDX42 FLJ43179 RHELP RNAHP SF3b125 SF3B8 DDX42P |
| Molecular Weight(MW) | 103 kDa |
Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DDX42 antibody can be used for detection of DDX42 by ELISA at 1:1562500. DDX42 antibody can be used for detection of DDX42 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX42 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
