Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | DDX48 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX48, DDX48, MUK34, NUK34, NMP265, eIF4AIII |
| Product description | DDX48 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.1) Lee, S., et al., (2002) Cancer Lett. 184 (2), 197-206. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX48. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDX48 antibody can be used for detection of DDX48 by ELISA at 1:312500. DDX48 antibody can be used for detection of DDX48 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | C.elegansDogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF4A3 |
|---|---|
| Alternative Names | DDX48 DDX48 MUK34 NUK34 NMP265 eIF4AIII |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DDX48 antibody can be used for detection of DDX48 by ELISA at 1:312500. DDX48 antibody can be used for detection of DDX48 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX48 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
