Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | DDX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX5, p68, HLR1, G17P1, HUMP68 |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX5 encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. DDX5 consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.1) Guil, S., et al., (2003) Mol. Cell. Biol. 23 (8), 2927-2941. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DDX5 antibody can be used for detection of DDX5 by ELISA at 1:62500. DDX5 antibody can be used for detection of DDX5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX5 |
|---|---|
| Alternative Names | DDX5 p68 HLR1 G17P1 HUMP68 |
| Molecular Weight(MW) | 68 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DDX5 antibody can be used for detection of DDX5 by ELISA at 1:62500. DDX5 antibody can be used for detection of DDX5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
