Application
Western blot analysis of DDX5 antibody in T47D cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | DDX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68, DDX5, G17P1, HELR, HLR1 |
| Product description | DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.1) Ong S.E., Mittler G.Nat. Methods 1:119-126(2004) |
| Immunogen | This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 491-518 amino acids from the C-terminal region of human DDX5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DDX5 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX5 DEAD box protein 5 RNA helicase p68 DDX5 G17P1 HELR HLR1 |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of DDX5 antibody in T47D cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
