Application
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).| Product Name | DDX6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK |
| Product description | This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.1) Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009) |
| Immunogen | This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-375 amino acids from the Central region of human DDX6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX6 |
|---|---|
| Alternative Names | Probable ATP-dependent RNA helicase DDX6 ATP-dependent RNA helicase p54 DEAD box protein 6 Oncogene RCK DDX6 HLR2 RCK |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
