WB
Western blot analysis of DDX6 using anti-DDX6 antibody.The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of DDX6 using anti-DDX6 antibody.DDX6 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-DDX6 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of DDX6 using anti-DDX6 antibody.DDX6 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-DDX6 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.IP
IP analysis of DDX6 using anti-DDX6 antibody in HepG2 whole cell lysate. Western blot analysis of DDX6 using anti- DDX6 antibody.Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- DDX6 antibody in HepG2 whole cell lysate, Lane 3: anti- DDX6 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DDX6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of HepG2 cells using anti-DDX6 antibody.Overlay histogram showing HepG2 cells stained with anti-DDX6 antibody (Blue line). To facilitate intrDDX6llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to DDX6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human DDX6 recombinant protein (Position: Q56-P483). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX6 |
|---|---|
| Gene Synonyms | P54 RCK HLR2 IDDILF Rck/p54 |
| Gene Full Name | DEAD-box helicase 6 |
| Gene Summary | This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012] |
| Alternative Names | ATP dependent RNA helicase p54 DDX6 DEAD box protein 6 HLR2 Oncogene RCK P54 RCK |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of DDX6 using anti-DDX6 antibody.The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of DDX6 using anti-DDX6 antibody.DDX6 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-DDX6 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of DDX6 using anti-DDX6 antibody.DDX6 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-DDX6 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.
IP
IP analysis of DDX6 using anti-DDX6 antibody in HepG2 whole cell lysate. Western blot analysis of DDX6 using anti- DDX6 antibody.Lane 1: HepG2 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- DDX6 antibody in HepG2 whole cell lysate, Lane 3: anti- DDX6 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DDX6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of HepG2 cells using anti-DDX6 antibody.Overlay histogram showing HepG2 cells stained with anti-DDX6 antibody (Blue line). To facilitate intrDDX6llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
