Application
Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.| Product Name | DDX60 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60 |
| Product description | DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.1) Miyashita M, Oshiumi H, Matsumoto M, et al. DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. Mol. Cell. Biol. 2011; 31:3802-19. |
| Immunogen | DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60. The immunogen is located within amino acids 1140 - 1190 of DDX60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX60 |
|---|---|
| Alternative Names | DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60 DEAD box protein 60 |
| Molecular Weight(MW) | Predicted: 125, 185, 188 kDa Observed: 190 kDa |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms. |
Application
Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DDX60 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DDX60 Antibody is supplied in PBS containing 0.02% sodium azide. |
