Application
Immunofluorescence of DELE in human brain tissue with DELE antibody at 20 μg/mL.Application
Western blot analysis of DELE in rat brain tissue lysate with DELE antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | DELE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DELE Antibody: DELE, DELE, Death ligand signal enhancer |
| Product description | DELE Antibody: DELE is a recently identified DAP3-binding protein that is thought to be important in the induction of death receptor-mediated apoptosis. Transfected cells that stably express DELE were found to be susceptible to apoptosis induction by TNF-α and TRAIL, whereas reducing DELE expression by siRNA rescued these cells from apoptosis induction. Furthermore, the reduction of DELE expression also inhibited the activation of caspase-3, caspase-8 and caspase-9 following stimulation by TNF-α, anti-Fas, or TRAIL, indicating the importance of DELE in apoptosis mediated by death receptors.1) Harada T, Iwai A, Miyazaki T. Identification of DELE, a novel DAP-binding protein which is crucial for death receptor-mediated apoptosis induction. Apoptosis 2010; 15:1247-55. |
| Immunogen | DELE antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human DELE. The immunogen is located within the first 50 amino acids of DELE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| DELE antibody can be used for detection of DELE by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIAA0141 |
|---|---|
| Alternative Names | DELE Antibody: DELE DELE Death ligand signal enhancer |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of DELE in human brain tissue with DELE antibody at 20 μg/mL.
Application
Western blot analysis of DELE in rat brain tissue lysate with DELE antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | DELE antibody can be used for detection of DELE by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DELE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DELE Antibody is supplied in PBS containing 0.02% sodium azide. |
