Application
Western blot analysis of DEPD5 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate.Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgWB
The interactions between RagA, RagAK142L and RagAK142R and their GEF Ragulator and their GAP GATOR1 were tested, respectively.| Product Name | DEPD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 1) Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005) |
| Immunogen | This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids from the C-terminal region of human DEPD5. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DEPDC5 |
|---|---|
| Alternative Names | DEP domain-containing protein 5 DEPDC5 KIAA0645 |
| Molecular Weight(MW) | 181 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of DEPD5 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate.
Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig
WB
The interactions between RagA, RagAK142L and RagAK142R and their GEF Ragulator and their GAP GATOR1 were tested, respectively.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
