Application
Western blot analysis of DEPDC1B in K562 cell lysate with DEPDC1B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of DEPDC1B in human spleen tissue with DEPDC1B antibody at 5 μg/ml.WB
A: DEPDC1B(OM276950) is highly expressed in Hs578T, MDA-MB-231, and BT-549 cells; its expression is low in MCF10A, MDA-MB-157, and MDA-MB-468 cells.(Cited reference: DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway)WB
B: the results of Western blot analysis confirmed DEPDC1B(OM276950) knockdown efficiency in steady cell lines.(Cited reference: DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway)| Product Name | DEPDC1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The DEP domain-containing 1B (DEPDC1B) protein was initially identified as a radiation response biomarker in human lymphoblastoid cell lines (1). Like the related protein DEPDC1A, expression of DEPDC1B correlates with a poor prognosis in cancer patients (2,3). Studies have shown that DEPDC1B levels are upregulated in non-small cell lung cancer (NSCLC) and have an inverse correlation with patient survival. Ectopic expression of DEPDC1B in NSCLC activated Wnt/B-catenin signaling and enhanced cell migration and invasion while silencing DEPDC1B expression suppressed these traits (2).1) Niu N, Qin Y, Fridley BL, et al. Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines. Genome Res. 2010; 20:1482-92. |
| Immunogen | DEPDC1B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DEPDC1B. The immunogen is located within the last 50 amino acids of DEPDC1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DEPDC1B |
|---|---|
| Alternative Names | DEP domain-containing 1B DEP1B BRCC3 Breast cancer cell 3 XTP1 |
| Molecular Weight(MW) | Predicted: 58 kDa Observed: 57 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | DEPDC1B antibody is human specific. At least two isoforms are known to exist; this antibody will only detect the largest isoform. DEPDC1B antibody is predicted to not cross-react with DEPDC1A. |
Application
Western blot analysis of DEPDC1B in K562 cell lysate with DEPDC1B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of DEPDC1B in human spleen tissue with DEPDC1B antibody at 5 μg/ml.
WB
A: DEPDC1B(OM276950) is highly expressed in Hs578T, MDA-MB-231, and BT-549 cells; its expression is low in MCF10A, MDA-MB-157, and MDA-MB-468 cells.(Cited reference: DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway)
WB
B: the results of Western blot analysis confirmed DEPDC1B(OM276950) knockdown efficiency in steady cell lines.(Cited reference: DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway)| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DEPDC1B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DEPDC1B antibody is supplied in PBS containing 0.02% sodium azide. |
