Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-hDGKB-T787 Pab in mouse liver tissue lysate.| Product Name | DGKB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-beta, DGKB, DAGK2, KIAA0718 |
| Product description | Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified1) Caricasole, A., et al., J. Biol. Chem. 277(7):4790-4796 (2002). |
| Immunogen | This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DGKB |
|---|---|
| Alternative Names | Diacylglycerol kinase beta DAG kinase beta 90 kDa diacylglycerol kinase Diglyceride kinase beta DGK-beta DGKB DAGK2 KIAA0718 |
| Molecular Weight(MW) | 91 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-hDGKB-T787 Pab in mouse liver tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
