Application
Antibody used in WB on Human brain at 0.2-1 ug/ml.Application
Antibody used in WB on Mouse at 1:1000.| Product Name | DGKE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DGKE, DAGK6, DGK, DAGK5, NPHS7 |
| Product description | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.1) Epand, R.M., (2007) Biochemistry 46 (49), 14225-14231. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DGKE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DGKE |
|---|---|
| Alternative Names | DGKE DAGK6 DGK DAGK5 NPHS7 |
| Molecular Weight(MW) | 64 kDa |
Application
Antibody used in WB on Human brain at 0.2-1 ug/ml.
Application
Antibody used in WB on Mouse at 1:1000.| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DGKE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
