Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
DGKQ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).| Product Name | DGKQ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Diacylglycerol kinase theta, DAG kinase theta, Diglyceride kinase theta, DGK-theta, DGKQ, DAGK4 |
| Product description | Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid.The predicted 882-amino acid human DGKQ (DGK-theta) protein is 90% identical in sequence to the rat homolog. DGK-theta is comprised of a pleckstrin homology domain and the conserved DGK putative catalytic domain. In contrast to other DGK isotypes, DGK-theta contains 3 rather than 2 cysteine-rich zinc-binding domains, an N-terminal proline- and glycine-rich region, and a RAS-associating domain. Highest tissue expression in the rat is in the brain. Defects in eye-specific DAGK genes cause retinal degeneration in Drosophila; thus, DAGK genes are candidates for human eye disease.1) Pilz, A., et al., Genomics 26(3):599-601 (1995). |
| Immunogen | This DGKQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-942 amino acids from the C-terminal region of human DGKQ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DGKQ |
|---|---|
| Alternative Names | Diacylglycerol kinase theta DAG kinase theta Diglyceride kinase theta DGK-theta DGKQ DAGK4 |
| Molecular Weight(MW) | 101 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
DGKQ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
