Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.Application
Western blot analysis in Ramos cell line lysates (35ug/lane).Application
Western blot analysis of DGKZ using DGKZ Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2).| Product Name | DGKZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta, DGKZ, DAGK6 |
| Product description | DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.1) Hogan, A., et al., J. Biol. Chem. 276(28):26526-26533 (2001). |
| Immunogen | This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DGKZ |
|---|---|
| Alternative Names | Diacylglycerol kinase zeta DAG kinase zeta Diglyceride kinase zeta DGK-zeta DGKZ DAGK6 |
| Molecular Weight(MW) | 124 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).
Application
Western blot analysis of DGKZ using DGKZ Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
