Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | DIAPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIAPH2, DIA |
| Product description | DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 amino acids from the Central region of human DIAPH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIAPH2 |
|---|---|
| Alternative Names | Protein diaphanous homolog 2 Diaphanous-related formin-2 DRF2 DIAPH2 DIA |
| Molecular Weight(MW) | 126 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
