Application
Western blot analysis of DIRAS3 in human testis tissue lysate with DIRAS3 antibody at 1 μg/ml.| Product Name | DIRAS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2 |
| Product description | DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).1) Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9. |
| Immunogen | DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3. The immunogen is located within the last 50 amino acids of DIRAS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIRAS3 |
|---|---|
| Alternative Names | DIRAS family GTP-binding RAS-like 3 ARHI NOEY2 |
| Molecular Weight(MW) | Predicted: 25 kDa Observed: 24 kDa |
| Tissue Specificity | DIRAS3 antibody is human specific. |
Application
Western blot analysis of DIRAS3 in human testis tissue lysate with DIRAS3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DIRAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide. |
